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. 2022 May 4;11(9):1541. doi: 10.3390/cells11091541

Figure 2.

Figure 2

Expression and localization of MD3. (A) Representative Western blots and densitometric analysis of MD3 variants in biopsies from the sigmoid colon of control, CD, and UC patients. MD3 variant 1 (v1, upper band, ~46 kDa) was upregulated in UC, whereas variant 2 (v2, lower band, ~45 kDa) did not change (* p < 0.05; n = 8 for each group, normalized for β-actin). (B) Representative immunofluorescence images from colon tissue of control, CD, and UC patients to determine MD3 localization. Signal intensity was increased for better visibility and therefore could not be used for quantitative estimations. No changes in MD3 localization (magenta) were detected between controls, CD, and UC patients (counterstain ZO-1, green). Bars = 20 µm. Right images are insets of shown merge images, with higher magnification. Bar = 10 µm.