TABLE 3.
Application of the method for quantifying the HAV genome to shellfish samples contaminated via natural routes (seven samples of mussels used)
| Virus extraction buffer (concentration process)a | Genome copies
|
||
|---|---|---|---|
| Mean no./gb | Mean log no./gb | SD (log no./g) | |
| Glycine (OF) | 9.38 × 105 | 5.97 | 0.45 |
| Glycine (OF, LH20) | 1.00 × 106 | 6.00 | 0.40 |
| Borate (OF) | 3.14 × 105 | 5.50 | 0.14 |
| Borate (PEG 6000) | 1.00 × 105 | 5.00 | 0.31 |
| Glycine (PEG 6000) | 5.48 × 104 | 4.74 | 0.22 |
| Borate (OF, LH20) | 5.38 × 104 | 4.73 | 0.09 |
| Glycine (PEG 6000, LH20) | 5.07 × 104 | 4.71 | 0.19 |
a
OF, organic flocculation; PEG 6000, viral precipitation using polyethylene glycol 6000; LH20, detoxification of samples by filtration on Sephadex LH20 gel.
b
Number of copies and log number of copies of HAV genome per gram of shellfish tissue were calculated with reference to last positive dilution of sample. Means of three independent quantification experiments on this last dilution are shown.