Skip to main content
. Author manuscript; available in PMC: 2022 May 13.
Published in final edited form as: Nat Biomed Eng. 2021 Apr 19;5(12):1485–1499. doi: 10.1038/s41551-021-00709-w

Fig. 7 |. The LINC complex promotes myofibroblast persistence.

Fig. 7 |

a, Schematic of the effect of DNKASH expression on the connection between the cytoskeleton and nuclear membrane. b, CCP of control-infected (mCherry) and DNKASH persistent myofibroblasts (7 d stiff) normalized to transient (1 d stiff) myofibroblasts. Two-tailed Student’s t-test; n = 39 cells. c, HDAC activity of control-infected (mCherry) and DNKASH persistent myofibroblasts (7 d stiff) normalized to transient (1 d stiff) myofibroblasts. Two-tailed Student’s t-test; n = 3 (control) and 4 (DNKASH) hydrogels. d, Schematic of the cell culture experiment to test for myofibroblast persistence in cells overexpressing DNKASH. e, Representative images of persistent myofibroblasts with expression of mCherry or DNKASH (9 d stiff (top) and 7 d–2 d stiff-to-soft (bottom); n = 12 images per hydrogel). CellMask, red; αSMA, green; DAPI, blue. Scale bars, 50 μm. f, Per cent of persistent myofibroblasts with expression of control and DNKASH (7 d–2 d). Two-tailed Student’s t-test; n = 3 hydrogels. Data from three biologically independent replicates. ****P < 0.0001. Data reported as the mean ± s.e.m.