Figure 2.

Expression of circ-RHOJ.1, miR-124-3p, and NRG1 in myocardial cells after I/R injury. Expression levels of NRG1 (A), circ-RHOJ.1 (B), and miR-124-3p (C) were assessed with qRT-PCR assay in sham group and I/R group, ***p < 0.001 versus sham group. D – Western blot assay was carried out to measure NRG1 expression in sham group and I/R group. GAPDH was used as a reference. Relative protein expression level of NRG1 was analyzed based on the protein gray values, *p < 0.05 versus sham group. E – Immunofluorescence assay was used to analyze NRG1 expression in sham group and I/R group. Green indicates NGR1 and blue (DAPI) indicates nuclei. Magnification, 400×, scale bars, 10 µm. All the data are presented as mean ± SD (n = 3)