Figure 1.

Biofilm formation induced by neutrophil protein extract. Total proteins were obtained from neutrophils and HaCaT cells, and the protein concentration was determined by Bradford. A bacterial culture from overnight incubation was diluted 1:200 and cultured with different concentrations of the neutrophil’s protein extract (A) and HaCaT protein extract (B) and the amount of biofilm formation was determined by the Christensen’s et al. method. Asterisk * indicates a significant difference (p < 0.05) concerning the control (bacteria without protein extract). The statistical analysis was performed using a one-way ANOVA with a Tukey’s test.