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. 2022 Apr 30;23(9):4992. doi: 10.3390/ijms23094992

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Assay of protease inhibition in the biofilm formation kinetics in isolate 54HS. Proteinase-3 was added to the kinetics from the beginning, and in addition 5 mM PMSF (a proteinase-3 inhibitor) was incorporated to the kinetics at 6 or 10 h after the starting point of culture in the isolate 54HS. The abundance of biofilm was determined according to Christensen et al. sampling at different points of the kinetics. Asterisk * indicates a significant difference (p < 0.05) compared to the bacteria with proteinase-3 since the beginning. The statistical analysis was performed using a one-way ANOVA with Tukey’s test.