Figure 6.

In-vivo biofilm formation by proteinase-3. Catheters were inoculated with the healthy skin non-biofilm-forming isolate 54HS or with the RP62A strain (control, biofilm-forming phenotype). In one catheter 2 µg/mL trypsin was added, and in the other 25 ng/mL proteinase-3 was added. Subsequently, the catheters were inoculated in the back of mice for 7 days. The quantity of viable bacteria attached to the catheters was determined. Asterisks indicate a significant difference (* p < 0.05, ** p < 0.01, and *** p < 0.001). The statistical analysis was performed using a one-way ANOVA with Tukey’s test.