Skip to main content
. 2022 Apr 30;23(9):4992. doi: 10.3390/ijms23094992

Figure 7.

Figure 7

Neutrophils induce biofilm in non-biofilm-forming isolates. (A) Neutrophils (600 cells/mL) in the presence of different MOI (neutrophils:bacteria) of non-biofilm-forming isolates were grown in wells with RPMI 10% FBS for 24 h. Biofilm formation was determinate by Christensen’s et al. method. (B) Neutrophils (600 cells/mL) were cultured in the presence of different MOI (neutrophils:bacteria) of non-biofilm-forming 54HS isolate (healthy skin) under different conditions: bacteria (control); neutrophils with bacteria (neutrophil); neutrophils with bacteria treated with 2 U DNAse I (Neutrophils-DNAse); neutrophils with bacteria treated with 1X cocktail of proteases inhibitors (Neutrophil-inhibitor proteases). After 24 h of culture the biofilm formation was determinate by the method of Christensen et al. Asterisk * indicates a significant difference (p < 0.05). The statistical analysis was performed using a one-way ANOVA with Tukey’s test.