Figure 8.
NETs formation. (A) Control conditions for NETs formation: neutrophils grown in media without bacteria (media); neutrophils treated with phorbol 12-myristate 13-acetate (PMA) for induction of NETs; neutrophils treated with PMA and DNAse (PMA + DNAse 100 U). (B) Induction of NETs in neutrophils (0.25 × 106 cells /mL) grown in the presence of different MOI (neutrophils:bacteria, 1:0.01, 1:1, 1:10) of non-biofilm-forming S. epidermidis isolate 54HS or S. aureus USA300 strain, after 4 h of culture. (C) Percentage of NETs formation of panel A and B assay, considering the number of cells counted as 100%. Scale bar in red: 50 µm.