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. 2022 Apr 30;23(9):4992. doi: 10.3390/ijms23094992

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effect of DNAse in proteinase-3-induced biofilm. (A) Bacteria without treatment with proteinase-3, C; bacteria treated with proteinase-3 at 25 ng/mL (P-3); bacteria treated with proteinase-3 and with 2 U DNAse I (P-3/DNAse); bacteria treated with 2 U DNAse I (DNAse); bacteria treated with proteinase-3 and with 5 mM PMSF inhibitor (P-3/I); bacteria treated with proteinase-3, and 5 mM PMSF inhibitor and 2 U DNAse I (P-3/I//D). (B) Bacteria without treatment with proteinase-3, C; bacteria treated with proteinase-3 at 25 ng/mL (P-3); bacteria treated with proteinase-3 more 2 U DNAse I at different times (0, 2, 4, 6, 8, 10, and 12 h). The abundance of biofilm was determined according to Christensen et al. Asterisk * indicates a significant difference (p < 0.05) to the P-3. The statistical analysis was performed using a one-way ANOVA with Tukey’s test.