FIG. 4.

Hybridization of total RNA isolated from phage sk1-infected L. lactis subsp. cremoris LM0230, LOC735, or AB002 at 0, 5, 15, and 30 min postinfection with ³²P-labelled sk1 total DNA digested with EcoRV (a) or a ³²P-labelled 3.9-kb EcoRV fragment from the late-expressed region (b). The first lanes contain EcoRV-digested phage sk1 DNA used as a positive control. Arrows indicate the 16S and 23S rRNA bands. (c) Temporal transcription map of phage sk1 with early-, middle-, and late-expressed regions indicated; adapted from Chandry et al. (8) with permission from the publisher.