Figure 4.

Principle of MALBAC whole-genome amplification: (1) MALBAC first stage preamplification is initiated with the annealing of partially random primers. (2) Strand elongation is achieved using a DNA polymerase with strand displacement activity. (3,4) Amplification of the semi-amplicons produces full amplicons with complementary 3′ and 5′ ends. (5) Looping prevents the full amplicons from further amplification during the first stage PCR and they instead serve as the target during the second stage regular PCR.