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. 2022 Apr 19;23(9):4474. doi: 10.3390/ijms23094474

Table 2.

Kinetic and equilibrium constants calculated for a variety of clones of mAb to cTnI used as a tracer antibody paired up with 19C7 clone served as a capture antibody.

mAb Clone kon × 10−5, M−1s−1 koff × 104, s−1 KA × 10−7, M1 KD × 109, M1
4C2 3.03 ± 0.21 6.99 ± 0.91 4.34 ± 0.87 23.05 ± 4.61
16A11 5.12 ± 0.61 9.22 ± 1.01 5.55 ± 1.28 18.02 ± 4.14
M155 0.17 ± 0.02 24.7 ± 4.7 0.07 ± 0.02 1419 ± 397
MF4 2.48 ± 0.30 19.7 ± 3.7 1.26 ± 0.39 79.5 ± 24.6
8E10 1.67 ± 0.08 23.4 ± 3.5 0.72 ± 0.14 140 ± 28