Fig. 8.

HMGB1 is critical for VCP to activate P13K/AKT/mTOR pathway in HCC cells. A, B The wound-healing assay was operated to test the moveable ability of Huh7 and MHCC-LM3 cells with indicated treatment. C–F The transwell system was used to evaluate the cell’s invasive capacity. G, H The influence of HMGB1 expression on VCP triggering the PI3K/AKT/mTOR pathway was assessed by western blot. The representative results and quantitative analyzed data were shown. I The GSEA analysis showed that the mTOR pathway was enriched in HCC patients with high expression of HMGB1 from TCGA database. The median value of HMGB1 transcriptional expression in the HCC cohort was adopted to classify patients into high and low HMGB1 expressed groups. All *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001