Table 1.
Embryo survival (%) and recovery (%) in three different types of explant isolated from two holm oak embryogenic lines after preculture of embryos on medium containing sucrose and incubation in PVS2 solution for 15 min with (+LN) or without (−LN) and subsequent cryostorage in LN for one month.
| Developmental Stage of Somatic Embryos | Survival (%) |
Embryo Recovery (%) |
||||
|---|---|---|---|---|---|---|
| Line Q8 | −LN | +LN | Mean | −LN | +LN | Mean |
| NES | 100 ± 0.0 a | 88.3 ± 2.8 a | 94.1 a | 96.7 ± 2.8 a | 56.7 ± 2.8 b | 76.7 a |
| Globular-heart | 100 ± 0.0 a | 3.3 ± 2.8 b | 51.7 b | 66.7 ± 2.8 b | 3.3 ± 2.8 c | 35.0 b |
| Cotyledonary | 100 ± 0.0 a | 0.0 ± 0.0 b | 50.0 b | 3.3 ± 2.8 c | 0.0 ± 0.0 c | 1.65 c |
| Mean | 100 a | 30.5 b | 55.6 a | 30.0 b | ||
| ANOVA II | ||||||
| Cryostorage in LN (A) | p ≤ 0.001 | p ≤ 0.001 | ||||
| Explant (B) | p ≤ 0.001 | p ≤ 0.001 | ||||
| A × B | p ≤ 0.001 | p ≤ 0.01 | ||||
| Line E2 | −LN | +LN | Mean | −LN | +LN | Mean |
| NES | 100 ± 0.0 a | 80.0 ± 0.0 b | 90.0 a | 93.3 ± 2.8 a | 46.7 ± 2.8 c | 70.0 a |
| Globular-heart | 100 ± 0.0 a | 0.0 ± 0.0 c | 50.0 b | 66.7 ± 2.8 b | 0.0 ± 0.0 d | 33.4 b |
| Cotyledonary | 100 ± 0.0 a | 0.0 ± 0.0 c | 50.0 b | 3.3 ± 2.8 d | 0.0 ± 0.0 d | 1.65 c |
| Mean | 100 a | 26.7 b | 54.4 a | 15.6 b | ||
| ANOVA II | ||||||
| Cryostorage in LN (A) | p ≤ 0.001 | p ≤ 0.001 | ||||
| Explant (B) | p ≤ 0.001 | p ≤ 0.001 | ||||
| A × B | p ≤ 0.001 | p ≤ 0.01 | ||||
Each value represents the mean ± standard error of three replicates. LN: liquid nitrogen; ns: not significant; NES: nodular embryogenic structures. Values indicated by different letters are significantly different (at p = 0.05).