Table 2.

(a) Survival (%) of nodular embryogenic structures of holm oak embryogenic lines (Q8 and E2) after preculture on medium containing sucrose and incubation in PVS2 solution for 15 or 30 min, with subsequent immersion in LN. (b) Recovery (%) of nodular embryogenic structures of holm oak embryogenic lines (Q8 and E2) after preculture on medium containing sucrose and incubation in PVS2 solution for 15 or 30 min, with subsequent immersion in LN.

(a)
Embryogenic Line/Time in PVS2 (min)	Control PVS2−LN	Storage Time in LN (Months)
Q8		1	6	12
PVS2 15	96.7 ± 3.2	90.0 ± 5.6	56.7 ± 3.2	63.3 ± 3.2
PVS2 30	90.0 ± 5.6	80.0 ± 0.0	60.0 ± 5.6	66.7 ± 3.2
E2		1	6	12
PVS2 15	90.0 ± 5.6	66.7 ± 8.5	63.3 ± 11.6	66.7 ± 3.2
PVS2 30	60.0 ± 5.6	53.3 ± 8.5	43.3 ± 3.2	40.0 ± 5.6
ANOVA II
Genotype (A)	p ≤ 0.05	p ≤ 0.01	ns	ns
PVS2 time (B)	p ≤ 0.05	ns	ns	ns
A × B	ns	ns	ns	p ≤ 0.05
(b)
Embryogenic Line/Time in PVS2 (min)	Control PVS2−LN	Storage Time in LN (Months)
Q8		1	6	12
PVS2 15	93.3 ± 3.2	83.3 ± 8.5	53.3 ± 3.2	60.0 ± 0.0
PVS2 30	86.7 ± 6.4	50.0 ± 5.6	60.0 ± 5.6	56.7 ± 8.5
E2		1	6	12
PVS2 15	90.0 ± 5.6	56.7 ± 11.6	53.3 ± 6.4	60.0 ± 5.6
PVS2 30	60.0 ± 5.6	40.0 ± 0.0	40.0 ± 0.0	40.0 ± 5.6
ANOVA II
Genotype (A)	ns	ns	ns	ns
PVS2 time (B)	ns	p ≤ 0.05	ns	ns
A × B	ns	ns	ns	ns

Each value represents the mean ± standard error of three replicates. Controls were precultured on medium containing sucrose and vitrification treatment was applied, but the explants were not cryostoraged in liquid nitrogen (LN). ns: not significant.