Table 3.
Survival (%) and embryo recovery (%) of four holm oak embryogenic lines cryopreserved after preculture on medium containing sucrose and incubation in PVS2 solution for 15 min with (+LN) or without (−LN) subsequent immersion in LN for one month.
| Embryogenic Line | Survival (%) |
Embryo Recovery (%) |
||
|---|---|---|---|---|
| −LN | +LN | −LN | +LN | |
| Q8 | 86.7 ± 3.2 a | 80.0 ± 0.0 a | 80.0 ± 6.4 | 60.0 ± 5.6 |
| E2 | 86.7 ± 6.4 a | 56.7 ± 3.2 b | 80.0 ± 0.0 | 43.3 ± 8.5 |
| E00 | 90.0 ± 0.0 a | 40.0 ± 5.6 c | 80.0 ± 0.0 | 36.7 ± 3.2 |
| Q10–16 | 86.7 ± 6.4 a | 46.7 ± 8.5 bc | 80.0 ± 9.6 | 43.3 ± 6.4 |
| Mean | 87.5 ± 0.7 a | 55.8 ± 7.6 b | 80.0 ± 0.0 a | 45.8 ± 4.3 b |
| ANOVA II | ||||
| Genotype (A) | ns | ns | ||
| Cryostorage in LN (B) | p ≤ 0.001 | p ≤ 0.001 | ||
| A × B | p ≤ 0.05 | ns | ||
Each value represents the mean ± standard error of three replicates. LN: liquid nitrogen; ns: not significant. Values followed by different letters are significantly different (at p = 0.05).