Table 5.
Nuclear DNA content (nDNA) of cryopreserved (+LN) and non-cryopreserved (−LN) embryos and plantlets regenerated from the corresponding embryogenic lines (E2 and Q8) of Quercus ilex (mean ± standard error).
| Embryogenic Line |
Tissue | Treatment | Ploidy | DNA Index | nDNA Content (pg/2C) | CV (%) |
n | Statistics |
|---|---|---|---|---|---|---|---|---|
| E2 | ||||||||
| Embryos | −LN | 2C | 0.244 ± 0.002 | 2.22 ± 0.02 | 5.27 | 4 | ns | |
| +LN | 2C | 0.243 ± 0.008 | 2.12 ± 0.07 | 5.68 | 4 | ns | ||
| Plantlets | −LN | 2C | 0.237 ± 0.006 | 2.15 ± 0.06 | 5.19 | 4 | ns | |
| +LN | 2C | 0.247 ± 0.005 | 2.24 ± 0.04 | 5.41 | 2 | ns | ||
| Q8 | ns | |||||||
| Embryos | −LN | 2C | 0.216 ± 0.004 | 1.97 ± 0.04 | 5.27 | 4 | ns | |
| +LN | 2C | 0.219 ± 0.003 | 1.99 ± 0.03 | 5.68 | 4 | ns | ||
| Plantlets | −LN | 2C | 0.226 ± 0.004 | 2.06 ± 0.04 | 5.19 | 3 | ns | |
| +LN | 2C | 0.233 ± 0. 007 | 2.12 ± 0.07 | 5.41 | 3 | ns |
LN: liquid nitrogen; n: sample number; ns: not significant.