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. 2022 May 8;23(9):5251. doi: 10.3390/ijms23095251

Figure 7.

Figure 7

EurGNC is a nuclear-localized transcriptional regulator. (A) Subcellular localization of EurGNC and EurCGA1 proteins. Fluorescence signals of GFP were detected in tobacco leaf epidermal cells. Left panel, DAPI and GFP image; middle panel, bright field; and right panel, merging of GFP and bright field. Bar, 25 µm. (B) Yeast one-hybrid assay to detect whether EurGNC regulates downstream genes (EurGUN5 and EurLHCB3). AbA (100 ng/L) was used to repress the autoactivation. Empty pGADT7 (AD) and AbAi were used as negative controls, while pGADT7-p53 and pAbAi-53 were used as positive controls.