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. 2022 May 4;23(9):5124. doi: 10.3390/ijms23095124

Table 1.

Primary antibodies, dilutions, detection systems, and pretreatments for immunohistochemistry. cCaspase-3: cleaved caspase 3; CK: cytokeratin; MW: microwave; SARS-CoV-2 NP: severe acute respiratory syndrome coronavirus-2 nucleoprotein.

Antigen Pretreatment Dilution Clonality Supplier Catalog Number Positive Control
cCaspase-3 MW 800 W—Citrate Buffer pH 6.0 1:500 Monoclonal rabbit, clone 5A1E Cell signaling 9664 Lymphoid tissue
CK14 MW 800 W—Citrate Buffer pH 6.0 1:800 Polyclonal rabbit Invitrogen PA5-16722 Liver and esophagus
FOXJ1 MW 800 W—Citrate Buffer pH 6.0 1:300 Polyclonal rabbit LSBio LS-B951 Trachea
p73 MW 800 W—Citrate Buffer pH 6.0 1:1000 Monoclonal mouse, clone 5B1288 Novusbio NB 100-56674SS Trachea
SARS-CoV-2 NP MW 800 W—Citrate Buffer pH 6.0 1:16000 Monoclonal mouse, clone 5 SinoBiological 40143-MM05 SARS-CoV-2 infected lung