Figure 3.

Mt respiration (n = 6/group). Measurement of Mt function in cultured DRG neurons using the XF24 analyzer. The oxygen consumption rate was measured at the basal level and with the subsequent and sequential addition of oligomycin, FCCP, and rotenone + antimycin A (AA) to DRG neurons cultured from CD, HFD, and HFD + NR 300 mg/kg mice (A). Levels of oxygen consumption rate were normalized per 6000 cells. From the oxygen consumption rate, basal respiration (B), maximal capacity respiration (C) and maximal reserve capacity (D) were calculated. The significance was calculated by ANOVA multiple comparison post hoc Tukey analysis. The statistical significance is indicated in the figure.; DRG neurons from HFD-fed mice compared to DRG neurons from CD and HFD + NR 300 mg/kg fed mice. ** p < 0.01.