The expression of NANOG in MCF7 cells cultured in patient-derived scaffolds presents prognostic value. (A) Forest plot of the association between the expressions of individual genes in MCF7 cells growing in 84 patient-derived scaffolds (PDSs) and disease-free survival (DFS). Multivariable analyses (MVA) for each gene including ERα-status, grade, age, tumor size and lymph node metastasis was conducted, and HR (Cox proportional hazards regression), 95% confidence interval (lower and upper) and the corresponding p-value were calculated and plotted. (B) Kaplan-Meier plot illustrating the relationship between NANOG expression in MCF7 cells growing in PDSs and DFS (blue, low expression; red, high expression). A log-rank statistical test was applied to compare survival in different strata (M, median; Q1, first quartile; Q3, third quartile), the best cut-off and the p-value are shown (detailed information in Supplementary Table S7). (* p-value < 0.05).