Figure 5.

Induction of lower expression of several markers in ERα-negative MDA-MB-231 cells by growing in ERα-negative patient-derived scaffolds are related to the disease-free time of the donor patients. (A,D) Forest plots of the association between the expression of the individual genes in MDA-MB-231 growing in 14 ERα-negative patient-derived scaffolds (PDSs) (A) or 53 ERα-positive PDSs (D) and disease-free survival (DFS) of the patients whose tumors were used to generate the PDSs. Multivariable analyses (MVA) adjusted by ERα-status, grade, age, tumor size and lymph node metastasis was conducted for each gene, and Cox proportional hazards regression (HR), 95% confidence interval (lower and upper) and the corresponding p-value were calculated and plotted. (B,C) Kaplan-Meier plots illustrating the relationship between expression of genes FOSL1 or SOX2 expression in MDA-MB-231 cells growing in ERα-negative PDSs and DFS (blue, low expression; red, high expression). A log-rank statistical test was applied to compare survival in different strata (M, median; Q1, first quartile; Q3, third quartile), and the best cut-off and p-values are shown (detailed information in Supplementary Table S7). * p-value < 0.05, ** p-value < 0.01.