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. 2022 May 3;11(9):1528. doi: 10.3390/cells11091528

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

KLC2 belongs to the target genes of the LINC00152-driven ceRNA network. (A) KLC2, STK39 and PHF19 are significantly downregulated in HuH7^ΔLINC00152 compared to HuH7 control cells. (B) Western blot analysis confirmed the decrease in KLC2 expression in HuH7^ΔLINC00152 (C,D). Reduced KLC2 mRNA and protein levels were observed following LINC00152 siRNA-medicated knockdown in HuH7 and HepG2 cells (E,F). Increased KLC2 mRNA and protein levels following overexpression of LINC00152 in HLE cells. Data are presented as mean ± SD of 4 independent experiments. Welch’s t-test was used: * p < 0.05, ** p < 0.01, *** p < 0.001. Abbreviations: gGFP, guide RNA targeting GFP; gLINC, guide RNA targeting LINC00152; siNS, scrambled, nonsense siRNA; siLINC_2/_3, siRNA 2 and 3 specifically targeting LINC00152; C, control cells transfected with empty vector; LINC00152 OE, LINC00152 overexpression; OE, overexpression; rel., relative.