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. 2022 May 3;11(9):1528. doi: 10.3390/cells11091528

Figure 5.

Figure 5

KLC2 exerts oncogenic functions in human HCC. (A) KLC2 is expressed both in the cytoplasmic and nuclear compartments of HuH7 and HepG2 cells. (B) Efficient siRNA-mediated knockdown of KLC2 protein levels in HuH7 and HepG2 cells compared to siNS control transfected cells. (C,D) Reduced cell viability and clonogenicity of both HuH7 and HepG2 cells following specific siRNA-mediated knockdown of KLC2 compared to siNS transfected control. (E) Migratory capacity of HuH7 cells either treated with KLC2-specific or siNS control siRNAs. Data are presented as mean ± SD of 4 independent experiments; in 5B only the upper error bar is displayed for graphical appearance reason. Welch’s t-test in (B), and unpaired t-test in (C–E): * p < 0.05, ** p < 0.01, *** p < 0.001. Abbreviations: cyto, cytoplasmatic protein fraction; nucl, nuclear protein fraction; siNS, scrambled, nonsense siRNA; siKLC2_1/_2, siRNA 1 and 2 specifically targeting KLC2; rel., relative.