FIGURE 7.

Properties of spontaneous inhibitory postsynaptic currents (IPSCs) recorded from artificial synapses incorporating human GlyR α2 ASD variants. (A) Representative recordings of IPSCs from HEK293 cells expressing wild-type GlyR α2 and α2^N38K, α2^K213E, and α2^T269M variants at two temporal scales. (B–D) Mean 10–90% rise times, IPSC decay time constants and amplitudes. Each data point represents the global average of all well-isolated events recorded from a single cell. Means were tested for significance relative to WT using one-way ANOVA followed by Tukey’s post hoc test: *p < 0.05, ***p < 0.001. All results are tabulated in Table 3.