TABLE 1.

Cellular expression profiles of GlyR α2 ASD/DD variants expressed in HEK293 cells.

	Whole cell		Cell-surface
Construct	Relative expression	Normalized expression (%)	Relative expression	Normalized expression (%)
GlyR α2 wild-type	0.54 ± 0.03	100 ± 5	0.63 ± 0.08	100 ± 12
GlyR α2V–22L	0.49 ± 0.19	92 ± 36	0.32 ± 0.07	52 ± 12*
GlyR α2N38K	0.14 ± 0.03	26 ± 6**	0.07 ± 0.04	11 ± 7**
GlyR α2K213E	0.37 ± 0.13	68 ± 25	0.26 ± 0.05	42 ± 8*
GlyR α2T269M	0.46 ± 0.08	86 ± 15	0.19 ± 0.06	30 ± 9*

Relative expression reflects the expression values obtained for the GlyR variants in relation to levels of the control protein pan-cadherin. For normalized expression, expression of GlyR α2 variants is shown as a percentage of wild-type GlyR α2 subunit values (100%). p-values were calculated relative to wild-type GlyR α2 homomers using Student’s t-test (analysis of variance) and values below *p < 0.05 were considered significant, **p < 0.01. Values are displayed as means ± standard error of the mean (±SEM).