TABLE 2.
Properties of wild-type and mutant GlyRs measured using whole-cell patch-clamp electrophysiology in HEK293 cells.
| Construct | Imax (nA) | nH | EC50 (μM) | n |
| GlyR α2 wild-type | 8.9 ± 1.6 | 2.1 ± 0.1 | 141 ± 14 | 7 |
| GlyR α2N38K | 3.3 ± 0.7** | 2.8 ± 0.1 | 243 ± 12*** | 7 |
| GlyR α2K213E | 5.2 ± 0.7 | 2.3 ± 0.3 | 177 ± 9 | 7 |
| GlyR α2T269M | 2.1 ± 0.4*** | 1.4 ± 0.2 | 4.5 ± 1.7*** | 7 |
The averaged maximal currents (Imax), Hill coefficients (nH), and EC50 values in response to glycine activation are shown. p-values were calculated relative to wild-type GlyR α2 homomers using one-way ANOVA followed by Tukey’s post hoc test: **p < 0.01, ***p < 0.001.