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. 2022 May 13;20:208. doi: 10.1186/s12967-022-03412-9

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — Upregulated miR-758-5p promoted osteogenic and odontogenic differentiation. A Transfection efficacy was shown by qRT-PCR in the manner of relative miRNA expression in PDLSCs. B CCK-8 assay showed the impact of overexpression of miR-758-5p on proliferation. C The relative expression of ALP, DSPP, OSX, and RUNX2 in the mimic, mimic NC, inhibitor, and inhibitor NC group. D Western blot assay detected the expression of ALP, DSPP, RUNX2, and OSX when miR-758-5p was overexpressed or inhibited. E Statistic analysis of the grayscale of western blot and data were shown as the ratio of the target protein to GAPDH. F Immunofluorescence photos showed that the intensity (represented the expression of ALP) in mimic, mimic NC, inhibitor, and inhibitor NC groups. G Result of ALP staining. H Result of ARS staining. I Quantification of ARS staining. J qRT-PCR showed that miR-758-5p regulates LMBR1. K Western blot showed that miR-758-5p regulates LMBR1. n = 3, **P < 0.01, *P < 0.05. Scale bar, 100 μm