Table 1.
Brain fatty acid composition.
| Fatty Acid % Total | APOE3 | APOE4 | |||||
|---|---|---|---|---|---|---|---|
| HF VCD |
HF VCD FO |
HF VCD |
HF VCD FO |
Genotype | Intervention | Interaction | |
| Total n-3 PUFA | 11.6 ± 0.70 | 13.6 ± 0.40 | 11.8 ± 0.50 | 13.5 ± 0.50 | 0.9991 | 0.0022 | 0.8036 |
| 20:5 n-3 (EPA) | 0.03 ± 0.01 | 0.06 ± 0.03 | 0.03 ± 0.01 | 0.09 ± 0.03 | 0.9242 | 0.3191 | 0.2855 |
| 22:5 n-3 | 0.10 ± 0.05 | 0.07 ± 0.03 | 0.06 ± 0.02 | 0.04 ± 0.01 | 0.2796 | 0.3508 | 0.9236 |
| 22:6 n-3 (DHA) | 11.2 ± 0.50 | 13.5 ± 0.40 | 11.7 ± 0.50 | 13.4 ± 0.50 | 0.7449 | 0.0006 | 0.5554 |
| Total n-6 | 12.6 ± 0.40 | 11.1 ± 0.30 | 13.2 ± 0.60 | 11.5 ± 0.40 | 0.2726 | 0.0036 | 0.8050 |
| 18:2 n-6 | 0.43 ± 0.07 | 0.43 ± 0.04 | 0.39 ± 0.06 | 0.43 ± 0.05 | 0.7696 | 0.7616 | 0.6878 |
| 20:2 n-6 | 0.08 ± 0.03 | 0.10 ± 0.05 | 0.07 ± 0.02 | 0.09 ± 0.02 | 0.7328 | 0.6002 | 0.9964 |
| 20:3 n-6 | 0.21 ± 0.02 | 0.42 ± 0.03 | 0.23 ± 0.02 | 0.40 ± 0.05 | 0.9581 | <0.0001 | 0.5492 |
| 20:4 n-6 (AA) | 8.10 ± 0.39 b | 7.5 ± 0.28 b | 8.67 ± 0.50 | 7.88 ± 0.26 | 0.2471 | 0.0892 | 0.7692 |
| 22:4 n-6 | 3.23 ± 0.14 | 2.57 ± 0.07 | 3.07 ± 0.15 | 2.64 ± 0.15 | 0.7537 | 0.0005 | 0.3814 |
| 22:5 n-6 | 0.54 ± 0.09 | 0.02 ± 0.01 | 0.77 ± 0.10 | 0.03 ± 0.02 | 0.1017 | <0.0001 | 0.1236 |
| DHA:AA | 1.39 ± 0.05 | 1.79 ± 0.02 | 1.36 ± 0.03 | 1.76 ± 0.04 | 0.3425 | <0.0001 | 0.9501 |
| SFA | 35.5 ± 0.90 | 37.0 ± 0.60 | 36.9 ± 1.20 | 36.6 ± 0.80 | 0.5870 | 0.5145 | 0.3146 |
| 14:0 | 0.24 ± 0.04 | 0.09 ± 0.03 | 0.18 ± 0.05 | 0.10 ± 0.03 | 0.5783 | 0.0052 | 0.2971 |
| 16:0 | 14.70 ± 0.70 | 16.40 ± 0.40 | 17.30 ± 0.90 | 16.30 ± 0.70 | 0.1000 | 0.6241 | 0.0809 |
| 18:0 | 18.90 ± 0.50 | 19.60 ± 0.30 | 18.30 ± 0.60 | 19.40 ± 0.30 | 0.4033 | 0.0600 | 0.6637 |
| 20:0 | 0.41 ± 0.03 | 0.38 ± 0.02 | 0.33 ± 0.03 | 0.35 ± 0.04 | 0.0966 | 0.7664 | 0.4637 |
| 22:0 | 0.38 ± 0.05 | 0.25 ± 0.09 | 0.19 ± 0.06 | 0.25 ± 0.09 | 0.2206 | 0.7141 | 0.2283 |
| 24:0 | 0.78 ± 0.18 | 0.26 ± 0.13 | 0.53 ± 0.14 | 0.39 ± 0.11 | 0.6621 | 0.0285 | 0.1980 |
| MFA | 30.5 ± 0.8 | 30.0 ± 0.8 | 27.9 ± 1.4 | 30.2 ± 0.9 | 0.2229 | 0.1262 | 0.0984 |
| 16:1 n-9 | 0.22 ± 0.06 | 0.36 ± 0.05 | 0.37 ± 0.06 | 0.37 ± 0.06 | 0.1805 | 0.2057 | 0.2327 |
| 16:1 n-7 | 0.42 ± 0.01 | 0.48 ± 0.04 | 0.46 ± 0.04 | 0.50 ± 0.02 | 0.4596 | 0.1600 | 0.6908 |
| 18:1 n-9 | 21.20 ± 0.80 | 20.60 ± 0.40 | 19.30 ± 1.20 | 20.20 ± 0.40 | 0.1337 | 0.8116 | 0.3281 |
| 18:1 n-7 | 3.58 ± 0.16 | 3.77 ± 0.12 | 3.39 ± 0.13 | 3.59 ± 0.11 | 0.1694 | 0.1470 | 0.9972 |
| 20:1 n-9 | 3.04 ± 0.19 | 2.73 ± 0.16 | 2.21 ± 0.21 | 2.82 ± 0.31 | 0.1248 | 0.5351 | 0.0603 |
| 22:1 n-9 | 0.21 ± 0.03 | 0.18 ± 0.04 | 0.18 ± 0.02 | 0.18 ± 0.04 | 0.7174 | 0.6213 | 0.6748 |
| 24:1 n-9 | 1.96 ± 0.18 a | 1.90 ± 0.16 a | 1.14 ± 0.24 b | 2.03 ± 0.23 a | 0.1094 | 0.058 | 0.0345 |
| Total DMA | 9.82 ± 0.47 | 8.23 ± 0.51 | 8.61 ± 0.44 | 8.48 ± 0.72 | 0.9861 | 0.1982 | 0.1098 |
PUFA, polyunsaturated fatty acid; 20:5 n-3 EPA, eicosapentaenoic acid; 22:5 n-3, docosapentaenoic acid; 22:6 n-3 DHA, docosahexaenoic acid; 18:2 n-6,, linoleic acid; 20:2 n-6, eicosadienoic acid; 20:3 n-6, dihomo-gamma-linolenic acid; 20:4 n-6 AA, arachidonic acid; 22:4 n-6, adrenic acid; 22:5 n-6, docosapentaenoic acid; DHA:AA, docosahexaenoic acid to arachidonic acid ratio; SFA, saturated fatty acid; 14:0, myristic acid; 16:0, palmitic acid; 18:0, stearic acid; 20:0, eicosanoic acid; 22:0, docosanoic acid; 24:0, tetracosanoic acid; MUFA, monounsaturated fatty acid; 16:1 n-9, palmitoleic acid; 16:1 n-7, palmitoleic acid; 18:1 n-9, oleic acid; 18:1 n-7, cis-vaccenic acid; 20:1 n-9, 11-eicosenoic acid; 22:1 n-9, erucic acid; 24:1 n-9, nervonic acid. Brain fatty acid composition of experimental animals (n = 5/6 per group) Data is % of total fatty acids and mean value ± SEM. Two-way ANOVA; a,b denote significant differences as analysed via Benjamini–Hochberg FDR correction if interaction effect was established. Bold numbers show significant p values.