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. 2022 May 5;27(9):2958. doi: 10.3390/molecules27092958

Figure 4.

Figure 4

Shows an example of how the LLOQ and the LLOD were determined. Chromatogram (A) was included in order to provide a comparison and identify the peak responses at low concentrations. Chromatogram (B), with TMZ acid (320 nM) and ES8 (207 nM) with retentions times of 2.3 and 9.7 min, respectively, was estimated to be the LLOQ for both TMZ acid and ES8. Chromatogram (C) represented a one-in-two dilution of the LLOQ. Chromatogram (D), with TMZ acid and ES8 at the concentrations of 80 and 52 nM, respectively, was estimated to be the LLOD. Chromatogram (E) represented a one-in-two dilution of the LLOD concentration and clearly indicated the absence of both the TMZ and ES8 analytes. The analysis was carried out at a wavelength of 325 nm.