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. 2022 Apr 29;11(9):1205. doi: 10.3390/plants11091205

Table 3.

List of the SSR primers used and their calculated informativeness parameters.

Primer Sequence (5–3) Tm NTB NPB PIC Rp MI
Xgwm-16 Forward GCTTGGACTAGCTAGAGTATCATAC 62.8 2 2 0.50 1.96 1.00
Reverse CAATCTTCAATTCTGTCGCACGG
Xgwm-44 Forward GTTGAGCTTTTCAGTTCGGC 59.9 2 2 0.47 2.42 0.93
Reverse ACTGGCATCCACTGAGCTG
Xgwm-111 Forward TCTGTAGGCTCTCTCCGACTG 59.5 2 2 0.24 3.32 0.47
Reverse ACCTGATCAGATCCCACTCG
Xgwm-121 Forward TCCTCTACAAACAAACACAC 54.3 2 2 0.09 2.12 0.19
Reverse CTCGCAACTAGAGGTGTATG
Xgwm-271 Forward CAAGATCGTGGAGCCAGC 58.5 2 2 0.36 2.74 0.73
Reverse AGCTGCTAGCTTTTGGGACA
Xgwm-272 Forward TGCTCTTTGGCGAATATATGG 55.9 2 2 0.08 3.84 0.15
Reverse GTTCAAAACAAATTAAAAGGCCC
Xgwm-292 Forward TCACCGTGGTCACCGAC 59.3 2 2 0.34 3.14 0.67
Reverse CCACCGAGCCGATAATGTAC
Xgwm-296 Forward AATTCAACCTACCAATCTCTG 55.6 2 2 0.30 2.16 0.61
Reverse GCCTAATAAACTGAAAACGAG
Xgwm-301 Forward GAGGAGTAAGACACATGCCC 59.5 2 2 0.40 1.86 0.79
Reverse GTGGCTGGAGATTCAGGTTC
Xgwm-325 Forward TTTCTTCTGTCGTTCTCTTCCC 69.3 2 2 0.44 2.00 0.88
Reverse TTTTTACGCGTCAACGACG
Xgwm-349 Forward GGCTTCCAGAAAACAACAGG 59.5 2 2 0.21 1.80 0.41
Reverse ATCGGTGCGTACCATCCTAC
Xgwm-382 Forward GTCAGATAACGCCGTCCAAT 59.2 2 2 0.33 2.28 0.67
Reverse CTACGTGCACCACCATTTTG
Xgwm-455 Forward ATTCGGTTCGCTAGCTACCA 56 2 2 0.36 2.46 0.73
Reverse ACGGAGAGCAACCTGCC
Xgwm-469 Forward CAACTCAGTGCTCACACAACG 63.5 2 2 0.23 2.00 0.45
Reverse CGATAACCACTCATCCACACC
Xgwm-515 Forward AACACAATGGCAAATGCAGA 60 2 2 0.32 3.06 0.64
Reverse CCTTCCTAGTAAGTGTGCCTCA
Xgwm-565 Forward GCGTCAGATATGCCTACCTAGG 62.1 2 2 0.46 2.40 0.92
Reverse AGTGAGTTAGCCCTGAGCCA
Xgwm-583 Forward TTCACACCCAACCAATAGCA 59.3 2 2 0.43 2.52 0.86
Reverse TCTAGGCAGACACATGCCTG
Xgwm-608 Forward ACATTGTGTGTGCGGCC 60.4 2 2 0.18 2.46 0.35
Reverse GATCCCTCTCCGCTAGAAGC
Xgwm-624 Forward TTGATATTAAATCTCTCTATGTG 51.3 2 2 0.48 2.36 0.97
Reverse AATTTTATTTGAGCTATGCG
Xgwm-157 Forward GTCGTCGCGGTAAGCTTG 60 2 2 0.46 1.98 0.93
Reverse GAGTGAACACACGAGGCTTG
Xgwm-212 Forward AAGCAACATTTGCTGCAATG 60 2 2 0.30 2.96 0.59
Reverse TGCAGTTAACTTGTTGAAAGGA
Xgwm-232 Forward ATCTCAACGGCAAGCCG 55 2 2 0.21 3.50 0.43
Reverse CTGATGCAAGCAATCCACC
Xgwm-311 Forward TCACGTGGAAGACGCTCC 60 2 2 0.21 2.52 0.41
Reverse CTACGTGCACCACCATTTTG
Xgwm-484 Forward ACATCGCTCTTCACAAACCC 55 2 2 0.39 1.96 0.79
Reverse AGTTCCGGTCATGGCTAGG
Mean 1.96 1.96 0.32 2.52 0.64

Tm, annealing temperature; NTB, number of total amplified bands; NPB, number of amplified polymorphic bands; PIC, polymorphism information content; Rp, resolving power; MI, marker index.