Table 3.
Primer | Sequence (5–3) | Tm | NTB | NPB | PIC | Rp | MI | |
---|---|---|---|---|---|---|---|---|
Xgwm-16 | Forward | GCTTGGACTAGCTAGAGTATCATAC | 62.8 | 2 | 2 | 0.50 | 1.96 | 1.00 |
Reverse | CAATCTTCAATTCTGTCGCACGG | |||||||
Xgwm-44 | Forward | GTTGAGCTTTTCAGTTCGGC | 59.9 | 2 | 2 | 0.47 | 2.42 | 0.93 |
Reverse | ACTGGCATCCACTGAGCTG | |||||||
Xgwm-111 | Forward | TCTGTAGGCTCTCTCCGACTG | 59.5 | 2 | 2 | 0.24 | 3.32 | 0.47 |
Reverse | ACCTGATCAGATCCCACTCG | |||||||
Xgwm-121 | Forward | TCCTCTACAAACAAACACAC | 54.3 | 2 | 2 | 0.09 | 2.12 | 0.19 |
Reverse | CTCGCAACTAGAGGTGTATG | |||||||
Xgwm-271 | Forward | CAAGATCGTGGAGCCAGC | 58.5 | 2 | 2 | 0.36 | 2.74 | 0.73 |
Reverse | AGCTGCTAGCTTTTGGGACA | |||||||
Xgwm-272 | Forward | TGCTCTTTGGCGAATATATGG | 55.9 | 2 | 2 | 0.08 | 3.84 | 0.15 |
Reverse | GTTCAAAACAAATTAAAAGGCCC | |||||||
Xgwm-292 | Forward | TCACCGTGGTCACCGAC | 59.3 | 2 | 2 | 0.34 | 3.14 | 0.67 |
Reverse | CCACCGAGCCGATAATGTAC | |||||||
Xgwm-296 | Forward | AATTCAACCTACCAATCTCTG | 55.6 | 2 | 2 | 0.30 | 2.16 | 0.61 |
Reverse | GCCTAATAAACTGAAAACGAG | |||||||
Xgwm-301 | Forward | GAGGAGTAAGACACATGCCC | 59.5 | 2 | 2 | 0.40 | 1.86 | 0.79 |
Reverse | GTGGCTGGAGATTCAGGTTC | |||||||
Xgwm-325 | Forward | TTTCTTCTGTCGTTCTCTTCCC | 69.3 | 2 | 2 | 0.44 | 2.00 | 0.88 |
Reverse | TTTTTACGCGTCAACGACG | |||||||
Xgwm-349 | Forward | GGCTTCCAGAAAACAACAGG | 59.5 | 2 | 2 | 0.21 | 1.80 | 0.41 |
Reverse | ATCGGTGCGTACCATCCTAC | |||||||
Xgwm-382 | Forward | GTCAGATAACGCCGTCCAAT | 59.2 | 2 | 2 | 0.33 | 2.28 | 0.67 |
Reverse | CTACGTGCACCACCATTTTG | |||||||
Xgwm-455 | Forward | ATTCGGTTCGCTAGCTACCA | 56 | 2 | 2 | 0.36 | 2.46 | 0.73 |
Reverse | ACGGAGAGCAACCTGCC | |||||||
Xgwm-469 | Forward | CAACTCAGTGCTCACACAACG | 63.5 | 2 | 2 | 0.23 | 2.00 | 0.45 |
Reverse | CGATAACCACTCATCCACACC | |||||||
Xgwm-515 | Forward | AACACAATGGCAAATGCAGA | 60 | 2 | 2 | 0.32 | 3.06 | 0.64 |
Reverse | CCTTCCTAGTAAGTGTGCCTCA | |||||||
Xgwm-565 | Forward | GCGTCAGATATGCCTACCTAGG | 62.1 | 2 | 2 | 0.46 | 2.40 | 0.92 |
Reverse | AGTGAGTTAGCCCTGAGCCA | |||||||
Xgwm-583 | Forward | TTCACACCCAACCAATAGCA | 59.3 | 2 | 2 | 0.43 | 2.52 | 0.86 |
Reverse | TCTAGGCAGACACATGCCTG | |||||||
Xgwm-608 | Forward | ACATTGTGTGTGCGGCC | 60.4 | 2 | 2 | 0.18 | 2.46 | 0.35 |
Reverse | GATCCCTCTCCGCTAGAAGC | |||||||
Xgwm-624 | Forward | TTGATATTAAATCTCTCTATGTG | 51.3 | 2 | 2 | 0.48 | 2.36 | 0.97 |
Reverse | AATTTTATTTGAGCTATGCG | |||||||
Xgwm-157 | Forward | GTCGTCGCGGTAAGCTTG | 60 | 2 | 2 | 0.46 | 1.98 | 0.93 |
Reverse | GAGTGAACACACGAGGCTTG | |||||||
Xgwm-212 | Forward | AAGCAACATTTGCTGCAATG | 60 | 2 | 2 | 0.30 | 2.96 | 0.59 |
Reverse | TGCAGTTAACTTGTTGAAAGGA | |||||||
Xgwm-232 | Forward | ATCTCAACGGCAAGCCG | 55 | 2 | 2 | 0.21 | 3.50 | 0.43 |
Reverse | CTGATGCAAGCAATCCACC | |||||||
Xgwm-311 | Forward | TCACGTGGAAGACGCTCC | 60 | 2 | 2 | 0.21 | 2.52 | 0.41 |
Reverse | CTACGTGCACCACCATTTTG | |||||||
Xgwm-484 | Forward | ACATCGCTCTTCACAAACCC | 55 | 2 | 2 | 0.39 | 1.96 | 0.79 |
Reverse | AGTTCCGGTCATGGCTAGG | |||||||
Mean | 1.96 | 1.96 | 0.32 | 2.52 | 0.64 |
Tm, annealing temperature; NTB, number of total amplified bands; NPB, number of amplified polymorphic bands; PIC, polymorphism information content; Rp, resolving power; MI, marker index.