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. 2022 May 7;23(9):5225. doi: 10.3390/ijms23095225

Figure 2.

Figure 2

Knockdown of Lmx1b enhances osteoblast formation. (a–c) Primary osteoblast precursor cells transfected with control siRNA (si-Control) or siRNA specific for Lmx1b (si-Lmx1b) were cultured in OGM. (a) Cells cultured for four days were analyzed for ALP activity. Data represent the means ± SD of triplicate samples. # p < 0.05 vs. si-Control. Results are representative of experiments that were independently repeated at least three times. (b) Cells cultured for six days were stained with Alizarin Red. Alizarin Red’s staining activity was quantified by densitometry at 570 nm. Data represent the mean ± SD of triplicate samples. * p < 0.05 vs. si-Control. Results are representative of experiments that were independently repeated at least three times. (c) Total RNA was isolated and mRNA expression levels of Runx2, Alpl, Ibsp, Bglap, and Lmx1b were determined using quantitative real-time PCR. Data represent the mean ± SD of triplicate samples. * p < 0.05, ** p < 0.001, # p < 0.005, vs. si-Control. Results are representative of experiments that were independently repeated at least three times.