Figure 2.

Circ_0085315 regulated cell proliferation, invasion, and migration as well as the expression change of Ki67, MMP2, E-cadherin, and N-cadherin in CC.

(A) circRNA_0085315 level was detected by qRT-PCR in si-con or si-circ_0085315 (Number 1 and 2) transfected SW620 cells. (B) The linear EIF3E level was detected by qRT-PCR in si-con or si-circ_0085315 (Number 1 and 2) transfected cells. (C) Evaluation of the viability exhibited was performed via the CCK-8 assay in cells transfected by si-con or si-circ_0085315. (D) Colony Formation Assay helped determine the impact of circ_0085315 silencing on colony formation ability. The transwell migration assay (E) and transwell invasion assay (F) helped determine the migration and invasion of SW620 cells and LOVO cells following the siRNA transfection. Western blot assay (G) and qRT-PCR assay (H) aimed at analyzing the expression of Ki67, MMP2, E-cadherin, and N-cadherin in CC cells under the transfection of si-con or si-circ_0085315 (Number 1 and 2). (B) In vector-con or vector-circ_0085315 transfected LOVO cells, circRNA_0085315 level (I), linear EIF3E level (J), cell viability (K), cell proliferation (L), cell invasion (M) and migration (N), and the expression of Ki67, MMP2, E-cadherin, and N-cadherin at protein level (O) and mRNA level (P) were detected by qRT-PCR, CCK-8, colony formation, Transwell, and Western blot assays. *P < 0.05; **P < 0.01.