Figure 3.

CircRNA_0085315 functioned as a ceRNA sponge to miR-1200.

(A) qRT-PCR assay was used to determine that the biotinylated circRNA_0085315 probe selectively brings down circRNA_0085315 level in SW620 and LOVO cells and not the expression of linear EIF3E. A total of 57 target miRNAs of circ_0085315 were shown in B based on Circular RNA Interactome database. (C, D, and E) RNA pulldown assays were conducted to examine the binding abilities between candidate miRNAs and circ_0085315 in SW620 and LOVO cells. (F) qRT-PCR assay was used to determine the biotinylated miR-1200 probe effectively reduced circRNA_0085315 level in SW620 and LOVO cells. (G) Relative miR-1200 expression in CC cell lines compared with HCoEpic by qRT-PCR analysis. (H) Relative miR-1200 expression in CC tumor tissues compared with adjacent normal tissues (n = 43) by qRT-PCR analysis. (I) Pearson correlation analysis of between circ_0085315 and miR-1200. *P < 0.05; **P < 0.01.