Figure 6.

MAP3K1 regulated miR-1200 impacting the biological behavior of CC cells and the activation of the MAPK signaling pathway.

(A) The transfection efficiency of MAP3K1 was verified using qRT-PCR and Western blot assays in SW620 and LOVO cells transfected by vector-con or vector-MAP3K1. Then, vector-MAP3K1 was transfected into miR-1200 mimics transfected cells, cell viability (B), proliferation (C), cell invasion (D), migration (E), and the phosphorylation level of JNK/p38/ERK1/2 were detected by CCK-8, colony formation, transwell, and Western blot assays. *P < 0.05; **P < 0.01.