Figure 7.

The regulatory network of circRNA_0085315/miR-1200/MAP3K1 functioned via the activation of the MAPK signaling pathway.

In SW620 cells transfected by miR-1200 mimics or si-MAP3K1, cell proliferation, invasion, migration, and the phosphorylation level of JNK/p38/ERK1/2 were detected by colony formation assay (A, B), CCK-8 (C), transwell invasion and migration assays (D, E, F, G). In LOVO cells transfected by vector-circ_0085315 and miR-1200 mimics/or si-MAP3K1, cell proliferation, invasion, migration, and the phosphorylation level of JNK/p38/ERK1/2 were detected by colony formation assay (H, I), CCK-8 (J), transwell invasion and migration assays (K, L, M, N), and Western blotting (O). *P < 0.05; **P < 0.01.