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Sensitivity of cancer cells to gemcitabine in PCO and CIPCO models. (A) Immunofluorescence detection of propidium iodide (PI) in PCOs and CIPCOs treated with various concentrations of gemcitabine (range: 0.1–10 μM). For the CIPCO, collagenase (100 ng/mL) was added. (green: live PCOs; red: dead cells). (B) Quantitative analysis of PI-positive areas. PI-positive areas were calculated using Gen5 Image Prime 3.08 (Biotek). Bar = 200 μm. *** p < 0.001.
