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Effect of the presence of TorD and effect of free nucleotides in the reconstitution mixture on the insertion of bis-Mo-MPT into apo-TorA. Here, 1.3 µM apo-TorA, 2 µM TorD or both were incubated with bis-Mo-MPT isolated from 30 µM YdhV, and 50 mM GMP, GDP or GTP was added prior to the reconstitution mixture. Bis-Mo-MPT was detected in form of its fluorescent oxidation product dephospho-FormA.
