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Bis-Mo-MPT content of 1.3 µM apo-TorA (A), apo-YdhV (B) and BSA (C) incubated with bis-Mo-MPT or dephospho-bis-MPT. Bis-Mo-MPT (circles) was directly isolated from YdhV. Dephospho-bis-Mo-MPT (triangles) was obtained after in vitro hydrolysis of the terminal bis-Mo-MPT phosphate group by alkaline phosphatase (AP). Bis-MPT and dephospho-bis-MPT were detected in form of their fluorescent oxidation product dephospho-FormA.
