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The O2 release at varying concentrations (0.0 mM, 0.625 mM, 1.25 mM, and 2.5 mM) of LAC at (a) pH 7.0, (b) pH 6.4, and (c) pH 6.0. The rate of O2 release was calculated from the linear portion of the graphs immediately after addition of LAC to oxy-Mb. Representative data from a single experiment per condition are shown here. All O2 kinetic experiments were repeated 3 times (n = 3).
