FIG. 2.

CDH activity in filtrates of P. cinnabarinus cultures grown on cellulose. Twenty-microliter aliquots of 10-fold-concentrated culture samples obtained from days 1 to 7 were separated by native PAGE (lanes 1 to 7, respectively). After electrophoresis, the gel was stained for CDH activity by using 2 mM DCPIP in 50 mM sodium tartrate (pH 4.0) and containing 2 mM cellobiose. Bands corresponding to CDH activity appear as clearing zones. The gel was scanned (Power Lock II; UMAX) and was labeled in Photoshop (Adobe) by using an Apple Power PC 9500 computer.