FIG. 3.

Separation of concentrated culture filtrate from cellulose-grown cultures of P. cinnabarinus by DEAE-M ion-exchange chromatography. Proteins were eluted with a linear 0 to 500 mM NaCl gradient in 25 mM sodium acetate (pH 5.0) (·····). The protein (——) and CDH activity (·——·) in the eluate were monitored.