TABLE 2.

Oxidative activities of P. cinnabarinus CDH I and CDH II^a

Substrate	Sp act (U/mg)
	CDH I	CDH II
Cellobiose	4.1 ± 0.5	15 ± 0.4
Lactose	1.2 ± 0.2	13 ± 0.3
Mannitol	0.3 ± 0.01	0.1 ± 0.02
Sorbose	0.3 ± 0.01	0.1 ± 0.01

^aEach reaction mixture (total volume, 1,000 μl) contained 50 mM sodium acetate (pH 4.5), 500 μM DCPIP, a water-soluble sugar substrate at a concentration of 2 mM, and 50 mg of a cellulose preparation. The assay mixtures were incubated at 25°C for 15 min. The results are means ± standard deviations based on triplicate measurements. No activity (DCPIP reduction after 12 h of incubation) was detected when any of the following compounds was the putative electron-donating substrate: bacterial cellulose, Whatman CF 11 cellulose, Avicel cellulose, d-glucose, d-xylose, and d-mannose.