TABLE 3.

Adsorption of P. cinnabarinus CDH I and CDH II to polysaccharides

Polysaccharide	Remaining activity in the supernatant (%)a
	CDH I	CDH II
Avicel cellulose	66	41
Bacterial cellulose	61	37
Whatman CF 11 cellulose	81	90
Xylan	102	96
Starch	103	99
Chitin	100	98
Control	100	100

^aCDH I (0.5 U/ml) or CDH II (0.5 U/ml) sodium was incubated with 1 mg of a polysaccharide in 50 mM sodium acetate (pH 4.5) in a 1,000-μl (total volume) reaction mixture at 25°C. After 1 h of incubation with constant agitation, the polysaccharides were removed by centrifugation, and the CDH activities in the supernatant were determined by using the standard DCPIP assay. Control reaction mixtures contained no polysaccharide. The values are the means based on duplicate values from three independent experiments; the maximum sample mean deviation from the values shown was 5%.