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. 2022 Apr 19;14(9):2050. doi: 10.3390/cancers14092050

Figure 2.

Figure 2

Treatment set-up. On the day before the start of the treatment, the cells of the respective cell line (either MDA-MB-231 or MCF-7) were seeded (displayed with the cell culture flasks). In hyperthermia-only treatment (HT), or HT followed by radiotherapy (HT + RT), HT treatment was performed on day 0 in the heating chamber system for 60 min with three different respective temperatures (39 °C, 41 °C, and 44 °C). After the HT treatment, irradiation was performed at the latest within 2 h for HT + RT. For RT + HT, the respective treatments were performed in the same manner but in reverse order. RT was performed in clinically relevant doses of 2 × 5 Gy. Irradiation in the respective RT + HT arms was always performed 2 h after the initial treatment at the latest. Sampling in all arms was performed on day 1 (24 h), d2 (48 h), d3 (72 h) and d5 (120 h) after the last irradiation.