Figure 3.

Gating strategy for the detection of cell death forms by AnnexinV/PI staining. Exemplarily shown are data of MCF-7 breast cancer cells. The cells were first gated on singlets (a,d) by FSC-A vs. FSC-H gating, followed by the exclusion of debris in the FSC/SSC plot (b,e). Viable cells were defined as Annexin negative/PI negative, apoptotic cells as Annexin positive/PI negative and necrotic cells as Annexin positive/PI positive (c,f). Data of cultured control samples (a–c) and of 2 × 5 Gy irradiated cells (d–f) are shown exemplarily.