Figure 10.

Expression of activation markers on moDCs after contact with hyperthermia- and radiotherapy treated MCF-7 breast cancer cells. Displayed is the expression of DC activation markers after 24 h (a)—CD83, (b)—CD70, (c)—CD80, and 48 h (d)—CD83, (e)—CD70, (f)—CD80 after co-incubation of immature moDCs with untreated MCF-7 tumor cells or with differently treated MCF-7 tumor cells. The tumor cells were treated with 2 × 5 Gy RT, HT of 44 °C, and first HT of 44 °C and then RT or RT followed by HT of 44 °C. As a positive control, immature moDCs were activated with the standard maturation cocktail (MC), and as negative control, immature moDCs were kept in moDC medium without the maturation cocktail (w/o MC). The expression of DC activation markers was analyzed by multicolor flow cytometry. The mean fluorescence intensity (ΔMFI) was calculated by subtracting the fluorescence intensity of unstained samples from stained samples. Mean ± SD are presented from at least four independent experiments. Statistical significance is calculated by using Kruskal–Wallis tests with Dunn’s correction by comparing the ΔMFI of the treatments to untreated controls at the corresponding timepoint, and Mann–Whitney U tests to compare the ΔMFI of different sequence of HT and RT. * (p < 0.1).The arm without maturation cocktail was compared to the arm with maturation cocktail with a Mann–Whitney U test # (p < 0.1), ## (p < 0.01).