| Component | Amount (μl) |
|---|---|
| Sample from Step 32 | 15.5 |
| FNK buffer | 6 |
| RNase inhibitor | 0.5 (20 U) |
| Turbo DNase | 1 (2 U) |
| FastAP | 3 (3 U) |
| T4 PNK | 4 (40 U) |
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