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. Author manuscript; available in PMC: 2022 May 13.
Published in final edited form as: Nat Protoc. 2021 Mar 26;16(4):2286–2307. doi: 10.1038/s41596-021-00501-9
Component Amount (μl)
Sample from Step 36 7
10× T4 RNA ligase buffer 2
DMSO (100%) 1.8
ATP (100 mM) 0.2
PEG 8000 (50%, wt/vol) 8
RNase inhibitor 0.3 (12 U)
T4 RNA ligase 1, high concentration 1.2 (36 U)